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DetailsCatalogue
Cell line:
T2
DSMZ no.:
ACC 598
Species:
human-human (Homo sapiens)
Cell type:
somatic cell hybrid
Origin:
established by PEG-mediated fusion of the B-lymphoblastoid cell line (B-LCL) LCL 721.174 with an 8-azaguanine and ouabain-resistant variant of the T-LCL CEM (CEMR.3); cells were described in the literature to synthesize HLA-A2 and -B5 RNA, but express only small amounts of A2 and no B5 antigen on the cell surface; subclone of the T1 cell line which has lost both CEMR-derived copies of chromosome 6; cell line also known as 174xCEM.T2
Reference(s):
14914, 14915
Biosafety level:
1
Risk assessment:
The cell line is positive for EBV (HHV-4) by PCR analysis. However, expression of immediate-early protein BZLF-1 and lately expressed capsid protein were negative by western blot and immunostaining, respectively, in untreated and phorbol ester / sodium butyrate stimulated cells. The infection was classified as latent infection without production of active viruses. A transmission of EBV during handling of the cells is unlikely and thus the cell line is categorized biosafety level 1.
Permissions and restrictions:
A
 
DSMZ Cell Culture Data:
Morphology:
round to polymorphic cells growing in suspension singly and in clumps
Medium:
90% RPMI 1640 + 10% h.i. FBS
Subculture:
seed out at ca. 0.2-1 x 106 cells/ml; split culture 1:2 to 1:4 every 2-3 days; maximal density at about 1.5 x 106 cells/ml
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 40-60 hours
Harvest:
cell harvest of ca. 1 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
 
DSMZ Scientific Data:
Mycoplasma:
negative in microbiological culture, PCR assays
Immunology:
CD2 -, CD3 -, CD4 +, CD5 -, CD6 -, CD7 +, CD8 -, CD10 -, CD13 -, CD19 +, CD20 +, CD34 -, CD37 +, CD38 -, CD138 -, TCRalpha/beta -, TCRgamma/delta -, HLA-DR -; image
Fingerprint:
fluorescent nonaplex PCR of short tandem repeat markers revealed a unique DNA profile
Species:
confirmed as human by species PCR
Cytogenetics:
human flat-moded hypertriploid karyotype with 5% polyploidy; 69-78<3n>XX, +2, +11, +13, +14, +16, +20, +20, +21, +mar, add(X)(q11), del(7)(q31.3), add(8)(p11), t(8;9)(p11;p24), del(9)(q21)x1-2, add(14)(q32)x1-2, add(16)(q11), add(18)(q23); derived from CCRF-CEM
Viruses:
PCR: EBV +, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV -
Supplied as:
Delivery form Prices
Frozen culture 360,- €
Growing culture
  (please inquire for exact delivery time)
 720,- €
DNA isolated from cell line (25 µg)  440,- €
DNA isolated from cell line (5 µg)  100,- €

see price list
 
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