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DetailsCatalogue
Cell line:
PEER
DSMZ no.:
ACC 6
Species:
human (Homo sapiens)
Cell type:
T cell leukemia
Origin:
established from the peripheral blood of a 4-year-old girl with T cell acute lymphoblastic leukemia (T-ALL) in second relapse in 1977; cell lines BE-13 (DSM ACC 396) and PEER show identical DNA fingerprints and hence share common origin, presumably due to cross-contamination; however, their diploid and tetraploid karyotypes indicative of earlier and later passage numbers, respectively, suggest that BE-13 is derived from PEER, rather than vice-versa; carries NUP214-ABL1 fusion gene
Reference(s):
14574, 14608, 14884, 15867, 15868, 15217
Biosafety level:
1
Permissions and restrictions:
A
 
DSMZ Cell Culture Data:
Morphology:
small, round cells growing mostly singly in suspension
Medium:
80% RPMI 1640 + 20% h.i. FBS
Subculture:
cells are very difficult to culture! cells grow best in 24-well-plates; maintain at ca. 0.5-1.0 x 106 cells/ml; split saturated culture 1:2 every 2-3 days; seed out at ca. 1.0 x 106 cells/ml (culture should not be split during the first 3-4 days after thawing)
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 50-60 hours
Harvest:
saturation density at 1.5 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
 
DSMZ Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology:
CD2 -, CD3 +, CD4 +, CD5 +, CD6 +, CD7 +, CD8 +, CD13 -, CD19 -, CD34 -, TCRalpha/beta -, TCRgamma/delta +; image
Fingerprint:
multiplex PCR of minisatellite markers revealed a unique DNA profile
Species:
confirmed as human with IEF of AST, NP
Cytogenetics:
human pseudodiploid karyotype with 7% polyploidy - 46(42-47)<2n>XX, der(4)?dup ins(4;4)(?p11;?q21q25), del(5)(q22q31), del(6)(q13q22), del(9)(p11p22), del(9)(q22) - resembles published karyotype - 5q-, 6q-, 9p- and 9q- associated with hematological malignancy
Molec. Genetics:
expression of NKX2-5 and MEF2C confirmed by RT-PCR and Western blot
Viruses:
ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV -
Supplied as:
Delivery form Prices
Frozen culture 360,- €
Growing culture
  (please inquire for exact delivery time)
 720,- €
DNA isolated from cell line (25 µg)  440,- €
DNA isolated from cell line (5 µg)  100,- €

see price list
 
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