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DetailsCatalogue
Cell line:
COS-7
DSMZ no.:
ACC 60
Species:
monkey - African green monkey (Chlorocebus aethiops (Cercopithecus aethiops))
Cell type:
kidney
Origin:
derived from CV-1, a simian cell line (cercopithecus aethiops), by transformation with an origin-defective mutant of SV-40; cells were described in the literature to support the growth of SV-40 viruses; classified as risk category 1 according to the German Central Commission for Biological Safety (ZKBS)
Reference(s):
14568
Biosafety level:
1, GMO-S1
Risk assessment:
The cell line was established by transfection with a mutated simian virus 40 (SV40) exhibiting a 6 bp deletion in the replication origin. This renders the virus incompetent for replication and the cell line can thus be handled under biosafety level 1.
Permissions and restrictions:
A, D, E
 
DSMZ Cell Culture Data:
Morphology:
fibroblast-like cells growing as monolayers
Medium:
90% Dulbecco's MEM + 10% h.i. FBS
Subculture:
split confluent culture 1:3 to 1:5 every 2-3 days using trypsin/EDTA; seed out at ca. 1 x 106 cells/80 cm2
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 35-48 hours
Harvest:
about 2-3 x 106 cells/80 cm2; saturation density at about 5 x 106 cells/80 cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
 
DSMZ Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Fingerprint:
unique Hinf I-(gtg)5 DNA profile (identical to COS-1, DSM ACC 63)
Species:
Chlorocebus aethiops (Cercopithecus aethiops) origin confirmed by COI DNA barcoding
Cytogenetics:
simian hypodiploid karyotype with 25% polyploidy - 57(53-58)<2n>
Viruses:
ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, SMRV -
Supplied as:
Delivery form Prices
Frozen culture 360,- €
Growing culture
  (please inquire for exact delivery time)
 720,- €
DNA isolated from cell line (25 µg)  440,- €
DNA isolated from cell line (5 µg)  100,- €

see price list
 
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