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DetailsCatalogue
Cell line:
KYO-1
DSMZ no.:
ACC 601
Species:
human (Homo sapiens)
Cell type:
chronic myeloid leukemia in blast crisis
Origin:
established from the peripheral blood of a 22-year-old Japanese man with chronic myeloid leukemia (CML) in myeloid blast crisis in 1981
Reference(s):
14814
Biosafety level:
1
Permissions and restrictions:
A
 
DSMZ Cell Culture Data:
Morphology:
round cells growing singly or in clumps in suspension; image
Medium:
80-90% RPMI 1640 + 10-20% h.i. FBS
Subculture:
split culture 1:2 to 1:4 every 2-3 days; seed out at ca. 0.5 x 106 cells/ml; maintain at ca. 0.2-0.8 x 106 cells/ml
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 40-50 hours
Harvest:
cell harvest of ca. 1 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
 
DSMZ Scientific Data:
Mycoplasma:
negative in microbiological culture, PCR assays
Immunology:
CD3 -, CD13 -, CD14 -, CD19 -, CD33 +, CD34 -, CD41 -, CD42b -, CD71 +, CD235a +; image
Fingerprint:
fluorescent nonaplex PCR of short tandem repeat markers revealed a unique DNA profile
Species:
confirmed as human by cytogenetics
Cytogenetics:
human flat-moded hypotetraploid karyotype; 83-90<4n>XX, -Y, -Y, +5, +8, -9, -9, +11, +11, +15, -16, -17, -17, -18, -18, +22, +22, +2mar; t(3;12)(p12.2;p11-12) x2, der(5)t(5;6)(p15;?q22)t(6;12)(q2?;?)ins(6;12)(q2?;?), del(6)(q22) x2, der(8)t(8;9)(q25;p11-12) x2, der(9)add(9)(p11-12)t(9;22)(q34;q11) x2, add(11)(q11) x2, der(12)t(6;12)(?;?) x2, der(15)t(15;22)(p11;q11) x2, der(17)t(17;17)(p12-13;q23) x2, der(22)t(9;22)(q34;q11) x4; carries t(9;22) effecting BCR-ABL1 rearrangement; resembles published karyotype
Molec. Genetics:
expression of fusion gene BCR-ABL1 e13-a2 (b2-a2) confirmed by RT-PCR
Viruses:
PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV -
Supplied as:
Delivery form Prices
Frozen culture 360,- €
Growing culture
  (please inquire for exact delivery time)
 720,- €
DNA isolated from cell line (25 µg)  440,- €
DNA isolated from cell line (5 µg)  100,- €

see price list
 
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