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DetailsCatalogue
Cell line:
RS-5
DSMZ no.:
ACC 604
Species:
human (Homo sapiens)
Cell type:
sarcomatoid mesothelioma
Origin:
established in 2003 from the untreated malignant mesothelioma of the pleura of a 78-year-old man with asbestos cancer
Reference(s):
14850
Biosafety level:
1
Permissions and restrictions:
A
 
DSMZ Cell Culture Data:
Morphology:
adherent, sarcomatoid-like cells growing in monolayers
Medium:
90% NCTC-109 + 10% h.i. FBS + 2 mM L-glutamine
Subculture:
split confluent culture 1:4 once a week using trypsin/EDTA; seed out at ca. 1-2 x 106 cells/175 cm2; cells grow slowly for about one week after thawing
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 4 days
Harvest:
maximum density of ca. 10 x 106 cells/175 cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
 
DSMZ Scientific Data:
Mycoplasma:
negative in microbiological culture, PCR assays
Immunology:
cytokeratin -, cytokeratin-7 -, cytokeratin-8 -, cytokeratin-17 -, cytokeratin-18 -, desmin -, endothel -, GFAP -, neurofilament -, vimentin +
Fingerprint:
fluorescent nonaplex PCR of short tandem repeat markers revealed a unique DNA profile
Species:
confirmed as human by cytogenetics
Cytogenetics:
human flat-moded near-diploid karyotype with 5% polyploidy; 46(39-48)<2n>XY; although no consistent abnormality was detected, most metaphases showed random chromosome loss
Viruses:
PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV -
Supplied as:
Delivery form Prices
Frozen culture 360,- €
Growing culture
  (please inquire for exact delivery time)
 720,- €
DNA isolated from cell line (25 µg)  440,- €
DNA isolated from cell line (5 µg)  100,- €

see price list
 
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