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DetailsCatalogue
Cell line:
SCC-25
DSMZ no.:
ACC 617
Species:
human (Homo sapiens)
Cell type:
squamous cell carcinoma
Origin:
established from the biopsy of a squamous cell carcinoma of the tongue of a 70-year-old man
Reference(s):
14889
Biosafety level:
1
Permissions and restrictions:
A
 
DSMZ Cell Culture Data:
Morphology:
epitheloid cells growing adherently in monolayers; image; image
Medium:
80% mixture of Ham's F12 + Dulbecco's MEM (at 1:1) + 20% h.i. FBS, 2 mM L-glutamine, 1 mM sodium pyruvate
Subculture:
seed out at ca. 1.0 x 106 cells/80 cm2; split confluent culture 1:5 to 1:10 once or twice a week using trypsin/EDTA (wash twice with PBS before trypsination)
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 50 hours
Harvest:
maximum density at ca. 5 x 106 cells/80 cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
 
DSMZ Scientific Data:
Mycoplasma:
negative in microbiological culture, PCR assays
Immunology:
cytokeratin +, cytokeratin-7 +, cytokeratin-8 +, cytokeratin-17 +, cytokeratin-18 +, cytokeratin-19 +, desmin -, endothel -, EpCAM +, GFAP -, neurofilament -, vimentin +
Fingerprint:
fluorescent nonaplex PCR of short tandem repeat markers revealed a unique DNA profile
Species:
confirmed as human by species PCR
Cytogenetics:
human highly rearranged hypertriploid karyotype with 4% polyploidy; 72-82<3n>XX, -Y, +4, -7, +9, +9, -13, -14, +15, -18, -19, -21, +10, -12 mar; add(1)(p31)x2, add(3)(?p11), add(4)(q11), i(5p), i(5q), der(8)t(1;8)(q25;p11)x1-2, del(9)(p11), i(9q), add(11)(q25), i(13q), add(14)(p11), add(15)(p11)x3-4, add(21)(q22)
Viruses:
PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HPV -, HTLV-I/II -, MLV -, SMRV -
Supplied as:
Delivery form Prices
Frozen culture 360,- €
Growing culture
  (please inquire for exact delivery time)
 720,- €
DNA isolated from cell line (25 µg)  440,- €
DNA isolated from cell line (5 µg)  100,- €

see price list
 
Print data sheet
Date of Collection
(in situ sampling):