293T
ACC 635
Cell line:
293T
DSMZ no.:
ACC 635
Species:
human (Homo sapiens)
Cell type:
embryonal kidney
Origin:
highly transfectable derivative of the human primary embryonal kidney cell line 293 (ACC 305) carrying a plasmid containing the temperature sensitive mutant of SV-40 large T-antigen (tsA1609); cells originally referred to as 293tsA1609neo; cells were described in the literature to be Geniticin (Neomycin, G418) resistant and to express SV-40 large T at 33°C, but not at 40°C; classified as risk group 1 according to the German Central Commission for Biological Safety (ZKBS)
Biosafety level:
1, GMO-S1
Risk assessment:
The cell line was established by transfection of the cells with sheared DNA of adenovirus type 5. The cells contain 4 to 5 copies of the left end of the virus (12% of the viral genome) including E1a and E1b genes and one copy of the right end of the virus (10% of the genome) including the E4 gene. No active viruses are produced.
Depositor-specific restrictions:
Transfer to third parties needs to be negotiated with the depositor.
DSMZ Cell Culture Data:
Medium:
90% DMEM + 10% h.i. FBS
Subculture:
seed out at ca. 1-3 x 106 cells/80 cm2; split confluent culture 1:4 - 1:5 every 2-3 days (without using trypsin/EDTA, cells can be detached by tapping the flask)
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 24-30 hours
Harvest:
cell harvest of ca. 40 x 106 cells/175 cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in microbiological culture, PCR assays
Immunology:
To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de.
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human flat-moded near-triploid karyotype; 61-73<3n>XXX, add(1)(p32), add(1)(q44)x1-2, der(4;10)(q10;q10), del(8)(p12), t(9;9)(p24;q21), der(13)t(6;13)(q21;q32), der(14)t(13;14)(q32;q32), del(17)(p11), der(22)t(17;22)(q21;q22)
Molec. Genetics:
presence of large T-antigene (SV40) was confirmed by genomic PCR
Viruses:
PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list