MDA-MB-453
ACC 65
Cell line:
MDA-MB-453
DSMZ no.:
ACC 65
Species:
human (Homo sapiens)
Cell type:
breast carcinoma
Origin:
established from an pericardial fluid of a 48-year-old woman with invasive breast carcinoma in 1976; described as ER and PR negative cell line; listed in the CCLE (ref 18232); RNA sequence data are available (see Ref 40906 and RNA-Seq)
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Medium:
80-90% Leibovitz's L-15 medium + 10-20% h.i. FBS
Subculture:
seed out at 2-4 x 106 cells/80 cm2; split confluent culture 1:2 to 1:6 using trypsin/EDTA; cells can partially be detached by tapping the flask; cells may need several days to re-adhere again
Incubation:
at 37 °C without CO2 gas exchange (no filter lid, lid of flask closed)
Doubling time:
ca. 50-60 hours
Harvest:
about 25-35 x 106 cells/80 cm2
Storage:
frozen with 85% medium, 10% FBS, 5% DMSO
DSMZ Scientific Data:
Mycoplasma:
initial contamination was eliminated with Ciprobay (ciprofloxacin), then negative in DAPI, microbiological culture, RNA hybridization, and continuously in PCR assays
Immunology:
To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de.
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human hypotetraploid karyotype with 6% polyploidy and near diploid sideline (2%) - 89(85-91)<4n>XXX, -X - highly rearranged leading to extensive marker formation, including der(5)t(5;?14)(p11;q11)x2 - dm present in 12% cells
Molec. Genetics:
Western blot: ER and PR negative, while AR and HER2 positive
Viruses:
ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
Delivery form | Prices | |
Frozen culture | 450,- € | |
Growing culture (please inquire for exact delivery time) | 900,- € | |
DNA isolated from cell line (25 µg) | 560,- € | |
DNA isolated from cell line (5 µg) | 140,- € |
see price list