EHEB
ACC 67
Cell line:
EHEB
DSMZ no.:
ACC 67
Species:
human (Homo sapiens)
Cell type:
chronic B cell leukemia
Origin:
established from the peripheral blood of a 69-year-old woman with B-CLL (chronic lymphocytic leukemia) prior treatment by EBV-transformation in 1988; cell line may represent rather a B-lymphoblastoid cell line than a bona fide B-CLL cell line
Reference(s):
Biosafety level:
1
Risk assessment:
The cell line is positive for EBV (HHV-4) by PCR analysis. However, expression of immediate-early protein BZLF-1 and lately expressed capsid protein were negative by western blot and immunostaining, respectively, in untreated and phorbol ester / sodium butyrate stimulated cells. The infection was classified as latent infection without production of active viruses. A transmission of EBV during handling of the cells is unlikely and thus the cell line is categorized biosafety level 1. According to a recommendation of the German Central Committee for Biological Safety (ZKBS) the cells have to be handled under biosafety level 2 when the cells are used in genetic engineering projects.
Permissions and restrictions:
DSMZ Cell Culture Data:
Morphology:
Medium:
90% RPMI 1640 + 10% h.i. FBS
Subculture:
seed out at ca. 0.5 x 106 cells/ml; cells may grow slowly initially; use at first 24-well plates and 20% FBS; during growth phase split at ca. 1:2 every 2-3 days and maintain at 0.1-0.5 x 106 cells/ml
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 50-70 hours
Harvest:
maximal density at about 1.0 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology:
CD3 -, CD10 -, CD13 -, CD19 +, CD20 +, CD34 -, CD37 +, CD38 +, cyCD79a +, CD80 +, HLA-DR +, cyIgG +, cyIgM -, cykappa +, cylambda -; image
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human hypodiploid karyotype with 1.6% polyploidy - 45(42-46)<2n>X, -X - no structural rearrangements detected
Viruses:
ELISA: reverse transcriptase negative; PCR: EBV +, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list