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DetailsCatalogue
Cell line:
CML-T1
DSMZ no.:
ACC 7
Species:
human (Homo sapiens)
Cell type:
T cell leukemia
Origin:
established from the peripheral blood of a 36-year-old woman with chronic myeloid leukemia (CML) in blast crisis in 1987; cells were described in the literature to express T cell surface markers and to carry the BCR-ABL1 translocation e13-a2 (b2-a2) producing the p210 BCR-ABL protein
Reference(s):
14972, 14699, 14479
Biosafety level:
1
Permissions and restrictions:
A
 
DSMZ Cell Culture Data:
Morphology:
single, round cells in suspension; image; image; image; image
Medium:
90% Iscove's MDM or RPMI 1640 + 10% h.i. FBS
Subculture:
maintain at 0.1-1.0 x 106 cells/ml; split 1:2 to 1:4 every 2-3 days; seed out at ca. 0.5 x 106 cells/ml in 24-well-plate
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 48-60 hours
Harvest:
maximal density at 2.5-3.0 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
 
DSMZ Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology:
CD2 -, cyCD3 +, CD3 (+), CD4 +, CD5 +, CD6 +, CD7 +, CD8 +, CD13 -, CD19 -, CD34 -, TCRalpha/beta (+), TCRgamma/delta -; image
Fingerprint:
multiplex PCR of minisatellite markers revealed a unique DNA profile
Species:
confirmed as human with IEF of AST, MDH, NP
Cytogenetics:
human near tetraploid karyotype with diploid sideline - 92<4n>XXX, t(6;7)(q24;q35)x2, del(11)(q22.3)x2
Molec. Genetics:
expression of fusion gene BCR-ABL1 (e13-a2) confirmed by RT-PCR
Viruses:
ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV -
Supplied as:
Delivery form Prices
Frozen culture 360,- €
Growing culture
  (please inquire for exact delivery time)
 720,- €
DNA isolated from cell line (25 µg)  440,- €
DNA isolated from cell line (5 µg)  100,- €

see price list
 
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