L-540
ACC 72
Cell line:
L-540
DSMZ no.:
ACC 72
Species:
human (Homo sapiens)
Cell type:
Hodgkin lymphoma
Origin:
established from the bone marrow of a 20-year-old woman with Hodgkin lymphoma (nodular sclerosis; stage IVB, pre-terminal stage)
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Morphology:
Medium:
80% RPMI 1640 + 20% h.i. FBS
Subculture:
seed out at ca. 0.5 x 106 cells/ml; after thawing culture first in 24-well-plates; cells do not grow during the first 2-3 days after thawing and viability may drop to 50-60%; in logarithmic growth split saturated culture 1:2 every 3 days, exchange spent medium every 1-2 days
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 70 hours
Harvest:
saturation density at about 1.5 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
initial contamination was eliminated with BM-Cyclin (tiamulin & minocycline), then negative in DAPI, microbiological culture, RNA hybridization, and continuously in PCR assays
Immunology:
CD2 +, CD3 -, CD13 -, CD14 -, CD15 +, CD19 -, CD21 -, CD25 +, CD30 +, CD34 -; image
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human flat-moded hypotriploid karyotype with 16% polyploidy - 59-64<3n>XX, -X, -1, +2, -6, +7, -8, -9, -10, -11, -13, -14, -16, -17, -18, +22, +5-6mar, der(1;5)(q10;p10)x1-2, der(2;12)(q10;q10), der(2)t(2;?8)(q3?7;q2?1)x1-2, ins(5;?)(p11;?), add(7)(p1?), add(8)(q24)x1-2, add(9)(p1?), add(11)(q24), der(15)t(1;15)(p21;p13)x2, add(19)(p11), add(21)(p13)
Molec. Genetics:
expression of fusion gene TNK1-TMEM256 (TNK1-C17orf61) confirmed by RT-PCR
Viruses:
ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list