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DetailsCatalogue
Cell line:
SK-BR-3
DSMZ no.:
ACC 736
Species:
human (Homo sapiens)
Cell type:
breast carcinoma
Origin:
established from the pleural effusion of a 43-year-old Caucasian woman with breast carcinoma in 1970
Reference(s):
17266
Biosafety level:
1
Permissions and restrictions:
A
 
DSMZ Cell Culture Data:
Morphology:
adherent cells growing in monolayers changing from round shape after seeding to epithelial-like cells later on; image
Medium:
80% McCoy´s 5a + 20% h.i. FBS
Subculture:
seed out at ca. 1-2 x 106 cells/80 cm2 flask; split semi-confluent culture 1:3 to 1:5 once or twice a week using trypsin/EDTA for 5-10 min
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 2-3 days
Harvest:
cell harvest of ca. 5 x 106 cells/80 cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
 
DSMZ Scientific Data:
Mycoplasma:
negative in microbiological culture, PCR assays
Immunology:
cytokeratin +, cytokeratin-7 +, cytokeratin-8 +, cytokeratin-17 -, cytokeratin-18 +, cytokeratin-19 +, desmin -, endothel -, EpCAM +, GFAP -, neurofilament -, vimentin -
Fingerprint:
fluorescent nonaplex PCR of short tandem repeat markers revealed a unique STR DNA profile
Viruses:
PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV -
Supplied as:
Delivery form Prices
Frozen culture 360,- €
Growing culture
  (please inquire for exact delivery time)
 720,- €
DNA isolated from cell line (25 µg)  440,- €
DNA isolated from cell line (5 µg)  100,- €

see price list
 
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