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DetailsCatalogue
Cell line:
BXPC-3
DSMZ no.:
ACC 760
Species:
human (Homo sapiens)
Cell type:
pancreas carcinoma
Origin:
established in 1980 from the biopsy specimen of the primary adenocarcinoma of the body of the pancreas from a 61-year-old woman; cells were described not to express cystic fibrosis transmembrane conductance regulator (CFTR)
Reference(s):
18730, 18731
Biosafety level:
1
Permissions and restrictions:
A
 
DSMZ Cell Culture Data:
Morphology:
epithelial-like cells growing adherently as monolayer in tight clusters or islands; single cells sometimes with long extensions; image; image; image
Medium:
80-90% RPMI 1640 + 10-20% h.i. FBS + 2 mM L-glutamine
Subculture:
seed out at ca. 2 x 106 cells/80 cm2 flask; split confluent culture 1:2 - 1:4 every second day using trypsin/EDTA
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 48 hours
Harvest:
cell harvest of ca. 10 x 106 cells/80 cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
 
DSMZ Scientific Data:
Mycoplasma:
negative in PCR assay
Immunology:
cytokeratin +, cytokeratin-7 +, cytokeratin-8 +, cytokeratin-17 +, cytokeratin-18 +, cytokeratin-19 +, desmin -, endothel -, EpCAM +, GFAP -, neurofilament -, vimentin (+)
Fingerprint:
genetic typing showed a pattern of short tandem repeats (STR) matching the reference profile of the international STR database
Viruses:
PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV -
Supplied as:
Delivery form Prices
Frozen culture 360,- €
Growing culture
  (please inquire for exact delivery time)
 720,- €
DNA isolated from cell line (25 µg)  440,- €
DNA isolated from cell line (5 µg)  100,- €

see price list
 
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