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DetailsCatalogue
Cell line:
PANC-1
DSMZ no.:
ACC 783
Species:
human (Homo sapiens)
Cell type:
pancreas carcinoma
Origin:
established in 1972 from the ductal cells of a tumor located in the head of the pancreas of a 56-year-old man; the cells are described in the literature to be tumorigenic in nude-athymic mouse, to carry three marker chromosomes and a small ring chromosome
Reference(s):
24155, 24156, 24157
Biosafety level:
1
Permissions and restrictions:
A
 
DSMZ Cell Culture Data:
Morphology:
epithelioid cells growing adherently as monolayer
Medium:
90% Dulbecco´s MEM + 10% h.i. FBS
Subculture:
seed out at ca. 1-2 x 106 cells/80 cm2 flask; split confluent culture 1:4 twice a week using trypsin/EDTA
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 40-45 hours
Harvest:
cell harvest of ca. 18-20 x 106 cells/175 cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
 
DSMZ Scientific Data:
Mycoplasma:
negative in PCR assay
Immunology:
cytokeratin-7 -, cytokeratin-8 +, cytokeratin-17 -, cytokeratin-18 +, cytokeratin-19 +, desmin -, endothel -, EpCAM -, GFAP -, neurofilament +, vimentin +
Fingerprint:
genetic typing showed a short tandem repeat (STR) profile indicated as unique by a search of the international STR database
Species:
confirmed as human by STR-typing
Viruses:
PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV -
Supplied as:
Delivery form Prices
Frozen culture 360,- €
Growing culture
  (please inquire for exact delivery time)
 720,- €
DNA isolated from cell line (25 µg)  440,- €
DNA isolated from cell line (5 µg)  100,- €

see price list
 
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