TMM
ACC 95
Cell line:
TMM
DSMZ no.:
ACC 95
Species:
human (Homo sapiens)
Cell type:
B lymphoblastoid cells (from patient with chronic myeloid leukemia in blast crisis)
Origin:
established from the peripheral blood of a 62-year-old man with Philadelphia chromosome negative chronic myeloid leukemia (CML) in blast crisis in 1985; cells were described in the literature to be Ph-negative, BCR-ABL1-negative; cells appear to be rather a "normal" B-lymphoblastoid cell line (B-LCL)
Reference(s):
Biosafety level:
1
Risk assessment:
The cell line is positive for EBV (HHV-4) by PCR analysis. However, expression of immediate-early protein BZLF-1 and lately expressed capsid protein were negative by western blot and immunostaining, respectively, in untreated and phorbol ester / sodium butyrate stimulated cells. The infection was classified as latent infection without production of active viruses. A transmission of EBV during handling of the cells is unlikely and thus the cell line is categorized biosafety level 1. According to a recommendation of the German Central Committee for Biological Safety (ZKBS) the cells have to be handled under biosafety level 2 when the cells are used in genetic engineering projects.
Permissions and restrictions:
DSMZ Cell Culture Data:
Medium:
90% Iscove's MDM + 10% h.i. FBS
Subculture:
seed out at 0.7 x 106 cells/ml; maintain at 0.2-0.8 x 106 cells/ml; split 1:1.5 to 1:2 every 2 to 3 days; the culture usually contains much cell debris
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 20-40 hours
Harvest:
saturation density at about 2.5-4.0 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, PCR assays
Immunology:
CD3 -, CD10 -, CD13 -, CD14 -, CD15 -, CD19 +, CD20 +, CD33 -, CD37 +, HLA-DR +, anti-EBNA2 +, sm/cyIgG -, sm/cyIgM -, sm/cykappa +, sm/cylambda -
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human near-diploid karyotype with 8% polyploidy - 46(42-46)<2n>XY - small ring present in 6% cells - no consistent numerical or structural rearrangement - resembles published karyotype
Viruses:
ELISA: reverse transcriptase negative; PCR: EBV +, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list