YAC-1
ACC 96
Cell line:
YAC-1
DSMZ no.:
ACC 96
Species:
murine (mouse) (Mus musculus)
Cell type:
lymphoma
Origin:
established by inoculation of the Moloney leukemia virus (MLV) into a newborn A/Sn mouse; cells were described in the literature to be sensitive to the cytotoxic activity of natural killer (NK) cells and are therefore often used as target cells in NK assays
Reference(s):
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Medium:
90% RPMI 1640 + 10% h.i. FBS
Subculture:
seed out at ca. 5 x 105 cells/ml, maintain at 0.3-2.0 x 106 cells/ml; cells grow very fast; split ratio of 1:5 to 1:10
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 20 hours
Harvest:
maximal density of about 3.0 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
contamination was eliminated with Mycoplasma Removal Agent, then negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Species validation:
Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Mus musculus species.
Cytogenetics:
hyperdiploid with 7% polyploidy - 41(38-42)<2n> - mouse chromosome morphology confirmed
Viruses:
ELISA: reverse transcriptase positive; PCR: SMRV -
Supplied as:
Delivery form | Prices | |
Frozen culture | 450,- € | |
Growing culture (please inquire for exact delivery time) | 900,- € | |
DNA isolated from cell line (25 µg) | 560,- € | |
DNA isolated from cell line (5 µg) | 140,- € |
see price list