Name:
Coleofasciculus chthonoplastes (Thuret ex Gomont 1892) Siegesmund et al. 2008
DSM No.:
104242 dsm-104242 dsm104242 dsm 104242 Coleofasciculus chthonoplastes
Other collection no.
or WDCM no.:
SAG 38.98,CCY 9606
Isolated from:
Intertidal habitat (marine); wadden sea
Country:
Germany
North Sea, Westhever, St. Peter Ording
Nagoya Protocol Restrictions:
There are NO known Nagoya Protocol restrictions for this strain.
History:
<- U. Karsten, Universität Rostock, Germany; {2014} <- St. Forster, Universität Rostock, Germany; STO {1995}
Genbank accession numbers:
16S + 23S partial sequence, intergenic spacer:
OR33940316S + 23S partial sequence, intergenic spacer:
EF654047.1
Cultivation conditions:
Medium 1673 , alternative medium: DSMZ Medium 1680, 23°C
At the DSMZ, the cyanobacterial cultures are usually cultivated without agitation in 25 cm2 plastic tissue culture flasks with filter screw caps. To subculture Coleofasciculus strains, scrape off the adhering cell material with a plastic Pasteur pipette and crush it by pipetting up and down several times. Alternatively, use a disposable pipette tip to dissect the cell material. Use 2-3 ml of the shredded biomass to inoculate 15 ml of fresh medium in a new tissue culture flask.
Light cycle: 16 hours of illumination at 150-200 lux and 8 hours dark period. Transfer interval of 12 weeks.
Complete DSMZ Media List
Summary and
additional information:
<- U. Karsten, Universität Rostock, Germany; {2014} <- St. Forster, Universität Rostock, Germany; STO {1995}. Intertidal habitat (marine); wadden sea. Germany, North Sea, Westhever, St. Peter Ording. Sequence accession no. 16S + 23S partial sequence, intergenic spacer: OR339403, 16S + 23S partial sequence, intergenic spacer: EF654047.1 . Phototrophic. It cannot be excluded that the strain produces cyanobacterial toxins that can be dangerous to humans and pets. DSM 104242 is a non-axenic culture. Strain-specific literature (27474). (Medium 1673, incubation time: more than 4 weeks, 23°C, alternative medium: DSMZ Medium 1680). At the DSMZ, the cyanobacterial cultures are usually cultivated without agitation in 25 cm2 plastic tissue culture flasks with filter screw caps. To subculture Coleofasciculus strains, scrape off the adhering cell material with a plastic Pasteur pipette and crush it by pipetting up and down several times. Alternatively, use a disposable pipette tip to dissect the cell material. Use 2-3 ml of the shredded biomass to inoculate 15 ml of fresh medium in a new tissue culture flask.
Light cycle: 16 hours of illumination at 150-200 lux and 8 hours dark period. Transfer interval of 12 weeks.
Risk group:
1 (classification according to German
TRBA)
Supplied as:
| Delivery form | | Prices |
|
| actively growing culture | | 160,- € | |
| Price Category for this culture: 1 |
Freight and handling charges will be added.
See price list.
