Name:
Polynucleobacter cosmopolitanus Hahn et al. 2010
DSM No.:
21490, Type strain dsm-21490 dsm21490 dsm 21490 Polynucleobacter cosmopolitanus
Strain designation:
MWH-MoIso2
Other collection no.
or WDCM no.:
CIP 109840,LMG 25212
Isolated from:
lake water
Country:
Austria
Lake Mondsee
Date of sampling:
15.07.2002
Nagoya Protocol Restrictions:
There are NO known Nagoya Protocol restrictions for this strain.
History:
<- M. W. Hahn, Austrian Academy of Sciences, Inst. Limnology; MWH-MoIso2
Cultivation conditions:
Medium 830 1:5 diluted, with cysteine and pyruvate (0.2 g/l each), keep agar surface moist, 28°C
Polynucleobacter strains grow so weakly that single colonies or a streak of colonies might not be visible on the agar surface. These bacteria grow reliably only if the agar surface is moist. We grow the cultures generally on agar slants with 1.5 -2 ml liquid media added. The slants are inoculated and incubated upright for about 1 week. The bacteria will grow and settle down as a faint white precipitate at the ground of the slant (deepest point of liquid). That is how the cultures are prepared for delivery also. Transfer about 250 µl of the suspension to inoculate fresh slants. Growth will be indicated by the precipitate.
Complete DSMZ Media List
Summary and
additional information:
<- M. W. Hahn, Austrian Academy of Sciences, Inst. Limnology; MWH-MoIso2. Lake water; Austria, Lake Mondsee. Type strain. Taxonomy/description (13757, 25987). Sequence accession no. AJ550672. (Medium 830 1:5 diluted, with cysteine and pyruvate (0.2 g/l each), 28°C). Polynucleobacter strains grow so weakly that single colonies or a streak of colonies might not be visible on the agar surface. These bacteria grow reliably only if the agar surface is moist. We grow the cultures generally on agar slants with 1.5 -2 ml liquid media added. The slants are inoculated and incubated upright for about 1 week. The bacteria will grow and settle down as a faint white precipitate at the ground of the slant (deepest point of liquid). That is how the cultures are prepared for delivery also. Transfer about 250 µl of the suspension to inoculate fresh slants. Growth will be indicated by the precipitate..
Risk group:
1 (classification according to German
TRBA)
Supplied as:
| Delivery form | | Prices |
|
| actively growing culture | | 160,- € | |
| DNA | | 150,- € | |
| Price Category for this culture: 1 |
Freight and handling charges will be added.
See price list.
