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BACTERIA
How to read the following data (Example)
Name:
Polynucleobacter difficilis Hahn et al. 2012
DSM No.:
22349, Type strain dsm-22349 dsm22349 dsm 22349 Polynucleobacter difficilis
Strain designation:
AM-8B5
Other collection no.
or WDCM no.:
CIP 110078
Isolated from:
freshwater taken from a depth of 60 m close to the bottom of a eutrophic lake
Country:
Armenia
Lake Sevan (40° 29' 35.50'' N 45° 11' 31.34'' E)
Date of sampling:
before 09.03.2009
Nagoya Protocol Restrictions:
There are NO known Nagoya Protocol restrictions for this strain.
History:
<- M. W. Hahn, Austrian Academy of Sci., Inst. Limnology; AM-8B5 <- A. Minasyan
Genbank accession numbers:
16S rRNA gene, IGS and 23S rRNA gene: FM208181
Cultivation conditions:
Medium 830 diluted 1:5. Add Na-pyruvate and cysteine-HCl x H2O, 0,2 g per L each, 25°C

Polynucleobacter strains grow so weakly that single colonies or a streak of colonies might not be visible on the agar surface. These bacteria grow reliably only if the agar surface is moist. We grow the cultures generally on agar slants with 1.5 -2 ml liquid media added. The slants are inoculated and incubated upright for about 1 week. The bacteria will grow and settle down as a faint white precipitate at the ground of the slant (deepest point of liquid). That is how the cultures are prepared for delivery also. Transfer about 250 µl of the suspension to inoculate fresh slants. Growth will be indicated by the precipitate. Incubation and delivery period several weeks

Incubation time: more than 14 days

Complete DSMZ Media List
Summary and
additional information:
<- M. W. Hahn, Austrian Academy of Sci., Inst. Limnology; AM-8B5 <- A. Minasyan. Freshwater taken from a depth of 60 m close to the bottom of a eutrophic lake; Armenia, Lake Sevan (40° 29' 35.50'' N 45° 11' 31.34'' E). Type strain. Taxonomy/description (16649). Sequence accession no. 16S rRNA gene, IGS and 23S rRNA gene: FM208181. (Medium 830 diluted 1:5. Add Na-pyruvate and cysteine-HCl x H2O, 0,2 g per L each, 25°C). Polynucleobacter strains grow so weakly that single colonies or a streak of colonies might not be visible on the agar surface. These bacteria grow reliably only if the agar surface is moist. We grow the cultures generally on agar slants with 1.5 -2 ml liquid media added. The slants are inoculated and incubated upright for about 1 week. The bacteria will grow and settle down as a faint white precipitate at the ground of the slant (deepest point of liquid). That is how the cultures are prepared for delivery also. Transfer about 250 µl of the suspension to inoculate fresh slants. Growth will be indicated by the precipitate. Incubation and delivery period several weeks.
For detailed information:
BacDive - The Bacterial Diversity Metadatabase
Literature:
16649
Risk group:
1 (classification according to German TRBA)
Supplied as:
Delivery form Prices
actively growing culture 125,- € 
Price Category for this culture: 1

Freight and handling charges will be added. See price list.
Other cultures:
All DSMZ cultures of the species