Pseudomonas phage Psp4
DSM 9285
PHAGES
Species:
Pseudomonas phage Psp4
Isolate:
Pseudomonas phage Psp4
DSM No.:
9285 dsm-9285 dsm9285 dsm 9285
History:
<- J. Campbell-Tofte, Copenhagen
Geographic origin:
country of origin unknown
Date of sampling:
before 16.06.1994
Nagoya Protocol Restrictions:
There are NO known Nagoya protocol restrictions for this strain.
Genomics:
240 kb
Morphology:
head diameter is 103 +/- 8 nm, extended tail length is 337 +/- 15 nm, appendages are tail fibres
Life cycle:
virulent phage, lytic cycle
Plaques description:
clear plaques are up to 1 mm in diameter
Image:
Single plaques on agar plate.
Creator: B. Henze, Leibniz Institute DSMZ.
© © Leibniz-Institut DSMZ
Further information:
For further general information see phage Psp1.
resistant to chloroform, < 2 minutes resistant to 60°C, the small burst size is 10 particles, the long latent period is 150 minutes.
Literature:
Risk group:
1
Phage propagation
Host bacterium:
Pseudomonas putida (Trevisan 1889) Migula 1895
DSM 9278
We strongly recommend to use only DSM 9278 for propagation.
DSM 9278
We strongly recommend to use only DSM 9278 for propagation.
Cultivation conditions:
Phage propagation:
Supply, storage and propagation of phages
Revitalisation of Vacuum-Dried Phages
Phage is not very productive, titer not higher than logE9 pfu/mL, plate propagation might be more efficient than liquid culture lysis. Optimal plate propagation at 25 °C for 2 days with 0.5% soft agar concentration.
Revitalisation of Vacuum-Dried Phages
Phage is not very productive, titer not higher than logE9 pfu/mL, plate propagation might be more efficient than liquid culture lysis. Optimal plate propagation at 25 °C for 2 days with 0.5% soft agar concentration.
Delivery
Supplied as:
| Delivery form | Prices | ||
| Liquid Suspension | 160,- € | ||
| Price Category for this culture: 6 | |||
Freight and handling charges will be added. See price list.