P-815
ACC 1
Cell line:
P-815
DSMZ no.:
ACC 1
Species:
murine (mouse) (Mus musculus)
Cell type:
mouse mastocytoma
Origin:
established from the mastocytoma tumor of a DBA/2 mouse treated with methylcolanthrene; used as target cells for cytotoxic T cell assays; as reported cells exhibit no effector activity in an antibody-dependent cell mediated cytotoxic system
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Morphology:
Medium:
90% RPMI 1640 + 10% h.i. FBS
Subculture:
seed out at ca. 0.5-1 x 106 cells/ml; starting in a 12- or 24-well plate may be of advantage;
maintain at 0.25-1.5 x 106 cells/ml; cells grow very fast; split 1:4 to 1:8 every 3-4 days
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 18-22 hours
Harvest:
maximum cell density at > 1 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Species validation:
Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Mus musculus species.
Cytogenetics:
murine near-diploid karyotype - 41(36-43)<2n> - 1-2 centric fusion markers present
Molec. Genetics:
genomic sequencing and/or ARMS assay show that the cell line carries the BRAF V600E mutation (mu/wt), but no NRAS Q61 mutation
Viruses:
ELISA: reverse transcriptase negative; PCR: SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list