C6-BU-1
ACC 108
Cell line:
C6-BU-1
DSMZ no.:
ACC 108
Species:
rat (Rattus norvegicus)
Cell type:
brain tumor
Origin:
this clone is a bromodeoxyuridine-resistant mutant derived from cell line C6 (established from a nitrosourea-induced brain tumor in a Wistar-Furth strain rat in 1971)
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Morphology:
Medium:
90% DMEM + 10% h.i. FBS + 100 µM 5-bromodeoxyuridine
Subculture:
split confluent cultures 1:4 to 1:10 every 4 days using trypsin/EDTA; let the cells not grow to complete confluence; seed out at ca. 4 x 106 cells/80 cm2
Incubation:
at 37 °C with 10% CO2
Doubling time:
about 30 hours
Harvest:
about 2-3 x 107 cells/80 cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Species validation:
Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Rattus norvegicus species.
Viruses:
ELISA: reverse transcriptase positive; PCR: SMRV -
Supplied as:
| Delivery form | Prices | |
| Growing culture (please inquire for exact delivery time) | 1040,- € | |
| Frozen culture | 520,- € | |
| DNA isolated from cell line (25 µg) | 650,- € | |
| DNA isolated from cell line (5 µg) | 160,- € | |
see price list