GM-7373
ACC 109
Cell line:
GM-7373
DSMZ no.:
ACC 109
Species:
bovine (Bos taurus)
Cell type:
endothelial aorta
Origin:
established from a male bovine fetus at 24-week gestation; vascular endothelium transformed with benzo(a)pyrene in vitro; cell line described as tumorigenic
Reference(s):
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Medium:
80% MEM (with Earle's salts) + 20% h.i. FBS + non-essential amino acids + MEM vitamins
Subculture:
split confluent culture 1:2 to 1:3 every 3-6 days using trypsin/EDTA; seed out at ca. 2 x 106 cells/80 cm2
Incubation:
at 37 °C with 5% CO2
Doubling time:
about 30-60 hours
Harvest:
cell harvest of about 0.8 x 105 cells/cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Species validation:
Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Bos taurus species.
Cytogenetics:
hypodiploid bovine karyotype with 15% polyploidy - 58(54-60)<2n> - centric fusion and large submetacentric marker - corresponds to published karyotype
Viruses:
ELISA: reverse transcriptase negative; PCR: SMRV -, BVDV +
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list