Species:
human (Homo sapiens)
Cell type:
erythroleukemia
Origin:
established from the peripheral blood of a 30-year-old man with erythroleukemia (AML M6) in relapse (after treatment for Hodgkin lymphoma) in 1980; cells were described in the literature to be capable of spontaneous and induced globin synthesis and to carry the JAK2 V617F mutation. Exome and RNA sequence data are available (see Ref 18187 and
Exome sequence and
RNA-Seq)
Permissions and restrictions:
Medium:
90% RPMI 1640 + 10% h.i. FBS
Subculture:
seed out at ca. 0.5 x 106 cells/ml; maintain at 0.2-1.0 x 106 cells/ml; split 1:3 to 1:5 every 3 days
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 36 hours
Harvest:
maximal cell density at 1.5 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
Mycoplasma:
initial contamination was eliminated with Ciprobay (ciprofloxacin), then negative in DAPI, microbiological culture, and continuously in PCR assays
Immunology:
CD3 -, CD13 +, CD14 -, CD19 -, CD33 +, CD41a +, CD71 +, CD235a +;
image
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human hypotriploid karyotype with 2.3% polyploidy - 63(60-64)<3n>XYY, -2, -9, -10, -10, -11, -14, -16, -16, -17, -19, +20, +21, +2mar, del(2)(q32), t(3;6)(p13;q16), der(5)t(5;17)(q10;q10), der(6)t(1;6)(p13;p21), der(7)add(7)(p14;q32), add(8)(p21), der(9)t(9;?)(?;11)(p24;?)(?;q13), del(11)(q13), add(15)(p11), del(20)(q13), r(20)(p11q11), dup(21)(q11q22.3-qter), psu dic(22;9)t(9;?)(?;22)(p24;?)(?;p11-13) - resembles published karyotype - carries also masked 5q- and 20q- consistent with AML M6/MDS
Molec. Genetics:
expression of mutated JAK2 confirmed by RT-PCR and sequencing
Viruses:
ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
| Delivery form | | Prices |
|
| Frozen culture | | 450,- € |
Growing culture
(please inquire for exact delivery time) | | 900,- € |
| DNA isolated from cell line (25 µg) | | 560,- € |
| DNA isolated from cell line (5 µg) | | 140,- € |
see price list
