Details - Leibniz Institute DSMZ

Cell line:

KOPM-88

DSMZ no.:

ACC 11029

Species:

human (Homo sapiens)

Cell type:

acute myeloid leukemia

Origin:

established in 1994 from the peripheral blood of an 11-year-old boy with acute myeloid leukemia in relapse; cell line carries t(X;11) and expresses KMT2A::SEPTIN6 fusion gene; the described partial tandem duplication in KMT2A was not detected

Reference(s):

41774, 41775

Biosafety level:

1

Permissions and restrictions:

A

DSMZ Cell Culture Data:

Morphology:

single round cells growing in suspension, some in small clumps; image; image; image; image

Medium:

80% RPMI 1640 + 10% h.i. FBS + 10% vol conditioned medium of cell line 5637 (DSMZ ACC 35)

Subculture:

seed out at ca. 0.5 x 10⁶ cells/ml; split 1:2 every 3-5 days; maintain at 0.2-0.5 x 10⁶ cells/ml; viability may drop after thawing for a few days

Incubation:

at 37 °C with 5% CO₂

Doubling time:

ca. 3-4 days

Harvest:

maximum cell density of ca. 1 x 10⁶ cells/ml

Storage:

frozen with 70% RPMI 1640, 20% FBS, 10% DMSO

DSMZ Scientific Data:

Mycoplasma:

negative in PCR assay

Immunology:

CD3-, CD4+, CD13+, CD14(+), CD15(+), CD19-, CD33+, CD34-, HLA-DR-, cyCD3-, cyCD68+-; image

Authenticity:

STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.

Species validation:

human

Cytogenetics:

t(X;11)(q24;q23)

Molec. Genetics:

KMT2A::SEPTIN6 fusion gene expression was confirmed by RT-PCR; positive for telomerase activity (by TRAP assay)

Viruses:

PCR: EBV-, HBV-, HCV-, HIV-1 -, HIV-2 -, HTLV-1/2 -, (X)MLV-

Supplied as:

Delivery form		Prices

Growing culture (please inquire for exact delivery time)		1040,- €
Frozen culture		520,- €
DNA isolated from cell line (25 µg)		650,- €
DNA isolated from cell line (5 µg)		160,- €

see price list

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