IEC-6
ACC 111
Cell line:
IEC-6
DSMZ no.:
ACC 111
Species:
rat (Rattus norvegicus)
Cell type:
normal small intestine
Origin:
normal rat epithelial cells from the small intestine; cells were described in the literature to synthesize fibronectin and collagen; no continuous cell line: about 10-15 doublings after thawing of the stock cells
Reference(s):
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Morphology:
Medium:
45% DMEM (4.5 g/L glucose) and 45% RPMI 1640 + 10% h.i. FBS + 0.1 U/ml insulin (human or rat)
Subculture:
split confluent cultures 1:2 to 1:6 about twice a week using trypsin/EDTA; seed out at ca. 1-2 x 106 cells/80 cm2
Incubation:
at 37 °C with 5% CO2
Doubling time:
about 50 hours
Harvest:
about 16 x 106 cells/175 cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Species validation:
Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Rattus norvegicus species.
Viruses:
ELISA: reverse transcriptase negative, SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list