XC
ACC 118
Cell line:
XC
DSMZ no.:
ACC 118
Species:
rat (Rattus norvegicus)
Cell type:
sarcoma
Origin:
the original tumor was induced in a Wistar rat by intramuscular injection of Rous sarcoma virus; cells have been used as indicator cells for detection of murine leukemia viruses
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Medium:
90% MEM (with Earle's salts) + 10% h.i. FBS + non-essential amino acids
Subculture:
split confluent culture 1:4 every 3 days using trypsin/EDTA
Incubation:
at 37 °C with 5% CO2
Harvest:
about 1-2 x 107 cells/80 cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization assays
Species validation:
Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Rattus norvegicus species.
Viruses:
ELISA: reverse transcriptase positive; PCR: SMRV -
Supplied as:
| Delivery form | Prices | |
| Growing culture (please inquire for exact delivery time) | 1040,- € | |
| Frozen culture | 520,- € | |
| DNA isolated from cell line (25 µg) | 650,- € | |
| DNA isolated from cell line (5 µg) | 160,- € | |
see price list