Species:
human (Homo sapiens)
Cell type:
chronic B cell leukemia
Origin:
established from the peripheral blood of a 63-year-old woman with B-prolymphocytic leukemia (B-PLL) at diagnosis; recent molecular biological characterization assigned this cell line to the entity "mantle cell lymphoma" (MCL); cell line was established by EBV-transformation during treatment with phorbol ester TPA; cells were described in the literature to have immunoglobulin light chain restriction (lambda) and to carry t(11;14) leading to IGH-CCND1 (BCL1) fusion gene and CCND1 activation. Exome and RNA sequence data are available (see Ref 18187 and
Exome sequence and
RNA-Seq)
Risk assessment:
The cell line is positive for EBV (HHV-4) by PCR analysis. However, expression of immediate-early protein BZLF-1 and lately expressed capsid protein were negative by western blot and immunostaining, respectively, in untreated and phorbol ester / sodium butyrate stimulated cells. The infection was classified as latent infection without production of active viruses. A transmission of EBV during handling of the cells is unlikely and thus the cell line is categorized biosafety level 1. According to a recommendation of the German Central Committee for Biological Safety (ZKBS) the cells have to be handled under biosafety level 2 when the cells are used in genetic engineering projects.
Permissions and restrictions:
Morphology:
lymphoblastoid, round to polygonal cells growing in clusters in suspension;
image;
image;
image;
image
Medium:
90% RPMI 1640 + 10% h.i. FBS
Subculture:
seed out at ca. 0.5 x 106 cells/ml; maintain at 0.1-0.5 x 106 cells/ml; cells might grow slowly after thawing; in log-phase split 1:2 to 1:4 every 2-4 days
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 50-70 hours
Harvest:
maximal density at about 1.5 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology:
CD3 -, CD10 -, CD13 -, CD19 +, CD20 +, CD34 -, CD37 +, CD38 +, cyCD79a +, CD80 +, CD138 +, HLA-DR +, cyIgG -, cyIgM +, cykappa -, cylambda +;
image
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human pseudodiploid karyotype with 1.8% polyploidy - 46(42-46)<2n>XX, t(1;15)(q32.3;q22.1), der(8)t(3;8)(q13;p21), t(11;14)(q13;q32), dup(16)(p11p13.2) - resembles published karyotype - carries t(11;14) associated with CCND1 (cyclin D1) activation
Molec. Genetics:
expression of fusion gene IGH-CCND1 (BCL1) confirmed by RT-PCR
Viruses:
ELISA: reverse transcriptase negative; PCR: EBV +, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
| Delivery form | | Prices |
|
| Frozen culture | | 450,- € |
Growing culture
(please inquire for exact delivery time) | | 900,- € |
| DNA isolated from cell line (25 µg) | | 560,- € |
| DNA isolated from cell line (5 µg) | | 140,- € |
see price list
