CHO-DHFR-
ACC 126
Cell line:
CHO-DHFR-
DSMZ no.:
ACC 126
Species:
Chinese hamster (Cricetulus griseus)
Cell type:
ovary cells
Origin:
subclone of the parental line CHO which was derived from the ovary of an adult Chinese hamster in 1957; the cells are dehydrofolate reductase negative (dhfr-; Dukx cells); cell line also termed CHO-DG44
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Medium:
90% alpha-MEM (with ribo- and deoxyribonucleosides) + 10% h.i. FBS + 2 mM L-glutamine (in case other media, e.g. DMEM or Iscove's MDM, are used, hypoxanthine and thymidine should be added as supplements)
Subculture:
seed out at ca. 0.5-2.0 x 106 cells/80 cm2; split confluent cultures 1:3 or higher every 2-4 days using trypsin/EDTA
Incubation:
at 37 °C with 10% CO2
Doubling time:
ca. 20-24 hours
Harvest:
about 10-20 x 106 cells/80 cm2 or 40-70 x 106 cells/175 cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, PCR assays
Species validation:
Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Cricetulus griseus species.
Cytogenetics:
near diploid hamster karyotype with 20% polyploidy - 21(20-22)<2n> - distinctive CHO morphology
Viruses:
ELISA: reverse transcriptase negative; PCR: SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list