CX-1
ACC 129
Cell line:
CX-1
DSMZ no.:
ACC 129
Species:
human (Homo sapiens)
Cell type:
colon adenocarcinoma (subclone of HT-29)
Origin:
cell line CX-1 was established from the human colon xenocraft model XC-1, which was established and propagated by inoculation of HT-29 cells into athymic mice; the HT-29 is also available from DSMZ (ACC 299)
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Morphology:
Medium:
90% DMEM + 10% h.i. FBS
Subculture:
split confluent culture 1:3 to 1:6 every 3-5 days using trypsin/EDTA, seed out about 106 cells/80 cm2
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 30 hours
Harvest:
about 30 x 106 cells/175 cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology:
To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de.
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an identical STR profile as HT-29 in the reference STR database.
Cytogenetics:
human hypertriploid karyotype with 14% polyploidy - 72(65-74)<3n>XX, -X, +4, +7, -8, -8, -9, +11, +11, -13, +15, +17, -18, +2mar, der(4)t(1;4)(p11;p15)t(1;?) (p32-36;?), del(4)(q32), del(7)(p21), i(13q), add(15)(p11), del(17)(p12), add(21)(p11) - two small markers present - carries 3 markers similar to those present in HT-29 - rearrangements of chs 1, 7, 17 typical of colon carcinoma
Viruses:
ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list