8305C
ACC 133
Cell line:
8305C
DSMZ no.:
ACC 133
Species:
human (Homo sapiens)
Cell type:
thyroid carcinoma
Origin:
established from the primary tumor of a 67-year-old woman with primary thyroid carcinoma (undifferentiated carcinoma, composed of spindle, polygonal and giant cells)
Reference(s):
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Medium:
80-90% RPMI 1640 + 10-20% h.i. FBS
Subculture:
seed out at about 0.5-1 x 106 cells/175 cm2; split confluent culture 1:5 once a week using trypsin/EDTA
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 55-60 hours
Harvest:
about 5-6 x 106 cells/175 cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology:
To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de.
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human flat-moded hypertriploid karyotype with 22% polyploidy - 74(67-76)<3n>XXX, -2, +9, -10, -12, -13, +14, +15, +15, +18, -19, +20, +4mar, 1-20 dm in most cells, der(6)t(6;7)(q22;q36q11.23), add(15)(p11)x2, del(17)(p12)x2 - subclonal rearrangements, e.g. del(2)(p16), del(2)(q22), del(3)(q22), i(4p), t(6p;7q), del(9)(p23)
Viruses:
ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list