MN-60
ACC 138
Cell line:
MN-60
DSMZ no.:
ACC 138
Species:
human (Homo sapiens)
Cell type:
B cell leukemia
Origin:
established in 1981 from the peripheral blood of a 20-year-old man with acute B cell leukemia of Burkitt's type (ALL FAB L3) in partial remission
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Medium:
90% Ham's F10 + 10% h.i. FBS
Subculture:
maintain at 0.2-0.5 x 106 cells/ml; seed out at ca. 0.5 x 106 cells/ml
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 48-60 hours
Harvest:
maximal density at 1.0 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology:
CD3 -, CD10 +, CD13 -, CD19 +, CD20 +, CD34 -, CD37 +, CD38 +, cyCD79a +, CD80 +, HLA-DR +, sm/cyIgG -, sm/cyIgM +, sm/cykappa -, sm/cylambda +; image
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human near diploid karyotype - 46(45-47)<2n>XY, dup(1)(q21q41), del(6)(q21), t(8;14)(q24;q32), i(13q)
Viruses:
ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
Delivery form | Prices | |
Frozen culture | 450,- € | |
Growing culture (please inquire for exact delivery time) | 900,- € | |
DNA isolated from cell line (25 µg) | 560,- € | |
DNA isolated from cell line (5 µg) | 140,- € |
see price list