
PLB-985 (subclone of HL-60)
ACC 139
Cell line:
PLB-985 (subclone of HL-60)
DSMZ no.:
ACC 139
Species:
human (Homo sapiens)
Cell type:
acute myeloid leukemia
Origin:
this cell line is a widely used subclone of cell line HL-60 with comparable but some different properties
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Medium:
90% RPMI 1640 + 10% h.i. FBS
Subculture:
seed out at ca. 1 x 106 cells/ml; maintain at ca. 0.5-1 x 106 cells/ml; split 1:2 to 1:5 every 2-3 days; minimum density at 0.2 x 106 cells/ml
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 30 hours
Harvest:
maximal density at about 1.5 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology:
CD3 -, CD4 +, CD13 +, CD14 -, CD15 +, CD19 -, CD33 +, CD34 -; image
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database, identical to that of cell line HL-60
Cytogenetics:
human near diploid karyotype with 4% polyploidy - 46(44-50)<2n>X, -X, +6, dm, dup(1)(q21q41), del(3)(p21), del(6)(q21)x2
Viruses:
ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
Delivery form | Prices | |
Frozen culture | 450,- € | |
Growing culture (please inquire for exact delivery time) | 900,- € | |
DNA isolated from cell line (25 µg) | 560,- € | |
DNA isolated from cell line (5 µg) | 140,- € |
see price list