Species:
human (Homo sapiens)
Cell type:
acute myeloid leukemia
Origin:
established from the bone marrow of a 59-year-old man with erythroleukemia that developed into acute myeloid leukemia (AML) at relapse in 1977; cells were described in the literature to form colonies in soft-agar in response to colony-stimulating factor and to form dendritic-like cells (DLC) in response to GM-CSF, IL-4, PMA (TPA), and ionomycin; sister cell line KG-1a. Exome and RNA sequence data are available (see Ref 18187 and
Exome sequence and
RNA-Seq)
Permissions and restrictions:
Medium:
90% RPMI 1640 + 10% h.i. FBS
Subculture:
seed out at ca. 1 x 106 cells/ml; maintain at 0.2-1.0 x 106 cells/ml; split 1:2 to 1:4 every 2-3 days
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 35-40 hours
Harvest:
maximum density at 1.0-1.5 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
Mycoplasma:
contamination was originally eliminated with Ciprobay (ciprofloxacin); later all tests ( DAPI, microbiological culture and PCR) were negative
Immunology:
CD3 -, CD13 +, CD14 -, CD15 +, CD19 -, CD33 +, CD34 +, HLA-DR +;
image
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human hypodiploid karyotype with 4.5% polyploidy - 45(42-47)<2n>X/XY, -4, +8, +8, -12, -17, -20, +2mar, der(5;17)(q10;q10)del(5)(q?11q?13), dup(7)(q12q33), del(7)(q22q35), i(8q)x2, der(8)t(6;8)(p11;q22), der(8)t(8;12)(p11;q13), der(11)t(1;11)(q13-21;p11-p13), der(16)t(?12;16)(?p13;q13/21) - resembles published karyotype
Molec. Genetics:
expression of fusion gene FGFR1OP2-FGFR1 (OP2-FGFR1) confirmed by RT-PCR
Viruses:
ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
| Delivery form | | Prices |
|
| Frozen culture | | 450,- € |
Growing culture
(please inquire for exact delivery time) | | 900,- € |
| DNA isolated from cell line (25 µg) | | 560,- € |
| DNA isolated from cell line (5 µg) | | 140,- € |
see price list
