
SK-HEP-1
ACC 141
Cell line:
SK-HEP-1
DSMZ no.:
ACC 141
Species:
human (Homo sapiens)
Cell type:
liver adenocarcinoma
Origin:
established from the ascites of a 52-year-old man in 1971 (with hepatocellular carcinoma)
Reference(s):
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Medium:
80-90% RPMI 1640 + 10-20% h.i. FBS
Subculture:
seed out at ca. 3-4 x 106 cells/80 cm2; split confluent culture 1:10 to 1:20 about 1-2 times a week using trypsin/EDTA; cells grow fast
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 30 hours
Harvest:
about 15-18 x 106 cells/175 cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
contamination was eliminated with Mycoplasma Removal Agent, then negative in DAPI, microbiological culture, RNA hybridization and PCR assays
Immunology:
To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de.
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human hypotriploid karyotype with 9% polyploidy - 62(60-63)<3n>X, -X, -X, -4, +6, +7, -9, +12, -13, -14, -15, -16, -18, der(1;7)(p10;q10), ?der(2;20)(p10;q10), der(6;13)(p10;q10)x2-3, add(12)(p1?), i(14q), add(19)(p13), add(21)(p11) - sideline with add(15)(p11) - resembles published karyotype
Viruses:
ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
Delivery form | Prices | |
Frozen culture | 450,- € | |
Growing culture (please inquire for exact delivery time) | 900,- € | |
DNA isolated from cell line (25 µg) | 560,- € | |
DNA isolated from cell line (5 µg) | 140,- € |
see price list