COLO-320
ACC 144
Cell line:
COLO-320
DSMZ no.:
ACC 144
Species:
human (Homo sapiens)
Cell type:
colon adenocarcinoma
Origin:
established from the tumor mass of a 55-year-old woman with a moderately undifferentiated adenocarcinoma of the sigmoid colon in 1977; cells were described in the literature to produce several hormones, e.g. serotonin, epinephrine; cells express MYC mRNA
Reference(s):
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Morphology:
Medium:
90% RPMI 1640 + 10% h.i. FBS
Subculture:
seed out at 1-3 x 106 cells in a T75 flask; split culture 1:2 to 1:4 every 2-4 days (trypsin/EDTA is not necessary as cells are only loosely adherent)
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 38 hours
Harvest:
maximal density at about 15 x 106 cells in a T75 flask
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
initial contamination was eliminated with Mycoplasma Removal Agent, then negative in DAPI, microbiological culture, RNA hybridization, and continuously in PCR assays
Immunology:
To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de.
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human hyperdiploid karyotype - 53(50-54)<2n>XX, -3, -3, +5, +7, +12, -13, -17, +18, +22, +6mar, t(1;5) (p11;q11), der(5)t(5;?)(p15;?), der(7)t(7;?)(q22;?), der(9)t(9;?)(p11;?), del(9)(p22), del(12)(p12), i(13q), der(14)t(14;?)(q24;?), der(18)t(18;?)(p11;?) - variable numbers of supernumerary dmins were present in 90% of cells
Viruses:
ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list