SP2/0-AG14
ACC 146
Cell line:
SP2/0-AG14
DSMZ no.:
ACC 146
Species:
murine (mouse) (Mus musculus)
Cell type:
myeloma
Origin:
SP2/0-AG14 is a hybrid between BALB/c spleen cells and the myeloma cell line P3X63AG8; cells were described as not synthesizing or secreting immunoglobulin chains, being resistant to azaguanine at 20 µg/ml, and not growing in HAT medium; cell line is used as a fusion partner for the production of hybridomas secreting specific monoclonal antibodies
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Medium:
92.5% Iscove's MDM + 7.5% h.i. FBS + 2 mM L-glutamine
Subculture:
maintain at 0.1-0.5 x 106 cells/ml; split 1:3 to 1:5 every 2-4 days; seed out at ca. 0.5 x 106 cells/ml; viability may be low after thawing and during the first few days, but cells recover after about one week
Incubation:
at 37 °C with 7.5% CO2
Doubling time:
ca. 30-40 hours
Harvest:
maximal density at 0.5-1.0 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Species validation:
Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Mus musculus species.
Viruses:
ELISA: reverse transcriptase positive; PCR: SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list