RBL-1
ACC 147
Cell line:
RBL-1
DSMZ no.:
ACC 147
Species:
rat (Rattus norvegicus)
Cell type:
basophilic leukemia
Origin:
this rat basophilic leukemia (RBL) cell line was established from chlorethylamine-treated rats; the cells were described in the literature to show marked variations in differentiation dependent on the growth rate and the cell cycle phases
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Morphology:
Medium:
90% RPMI 1640 + 10% h.i. FBS (cells may also be grown, but less efficiently, with MEM)
Subculture:
maximal split ratio of 1:4; split 1:3 every 2-3 days; cells grow partly adherent, but trypsin is not needed to detach the cells; seed out at ca. 5 x 106 cells/80 cm2 or 0.3-0.5 x 10 6 cells/ml
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 25 hours
Harvest:
about 46 x 106 cells/175 cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Species validation:
Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Rattus norvegicus species.
Cytogenetics:
74(69-76) - rodent marker chromosomes present
Viruses:
ELISA: reverse transcriptase negative; PCR: SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list