NEURO-2A
ACC 148
Cell line:
NEURO-2A
DSMZ no.:
ACC 148
Species:
murine (mouse) (Mus musculus)
Cell type:
neuroblastoma
Origin:
established from the spontaneous tumor of a strain A albino mouse
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Morphology:
Medium:
90% DMEM + 10% h.i. FBS + 1x non-essential amino acids
Subculture:
seed out at ca. 1 x 106 cells/25 cm2; split culture 1:4 about once a week using trypsin/EDTA, cells do not grow to confluence and grow rather slowly
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 70 hours
Harvest:
about 8 x 106 cells/75 cm2; maximal density at ca. 20 x 106 cells/175 cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Species validation:
Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Mus musculus species.
Viruses:
ELISA: reverse transcriptase positive; PCR: SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list