KB-V1
ACC 149
Cell line:
KB-V1
DSMZ no.:
ACC 149
Species:
human (Homo sapiens)
Cell type:
cervix carcinoma (derivative of HELA)
Origin:
KB-V1 is a multidrug-resistant (mdr) subclone derived in 1985 from KB-3-1 (DSMZ ACC 158) which itself is a derivative of the 1951 established HELA (DSM ACC 57) (31-year-old woman); cells express strongly mdr1 mRNA and protein
Biosafety level:
1
Risk assessment:
The cell line is infected with papilloma virus type 18 (HPV-18). The cells contain several copies as proviruses integrated into the eukaryotic genome. The integrated virus genomes are incomplete and exhibit 2-3 kb deletions of the E2-L2 region. An activation and transmission of the HPV-18 during handling of the cell line is improbable. The cell line is categorized biosafety level 1.
Permissions and restrictions:
Depositor-specific restrictions:
The cell line will only be distributed to non-profit institutions,
commercial entities may apply for a license at the NIH/NCI Technology Transfer Cernter, Rockville, MD, USA, phone (301)435-3111. Transfer to third parties needs to be negotiated with the depositor.
DSMZ Cell Culture Data:
Morphology:
Medium:
85% DMEM + 15% h.i. FBS + 200-1000 ng/ml vinblastine
Subculture:
cells are difficult to culture in the presence of vinblastine! split ratio of confluent cultures 1:3 to 1:10; split every 2-4 days, dependent on the vinblastine concentration, using trypsin/EDTA; upon thawing, first grow the cells with vinblastine, then in increasing amounts; seed out at ca. 3 x 106 cells/80 cm2 in 10 ml medium
Incubation:
at 37 °C with 10% CO2
Doubling time:
ca. 50 hours
Harvest:
cell harvest of about 6-10 x 106/80 cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology:
To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de.
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human flat-moded hypotriploid karyotype with 13% polyploidy - 71-77<3n>XX, -X, +1, +3, +5, +5, +5, +7, +7, +8, +8, -9, -11, +12, +15, -16, +17, -18, -19, +21, -22, +mar, der(1;3)(q10;p10), del(3)(q13), der(3;5)(p10;q10), i(5p)x3, del(7)(q10), der(7)add(7)(p1?)t(7;11)(q3?5;q11), add(8)(q12), del(9)(p24), add(9)(q34), add(12)(p13), add(12)(q1?4), add(15)(p11), add(22)(p11)x2 - drug selection maintains variable numbers of dmin visible in 26% cells as well as smaller particulate chromatin in most remaining cells - karyotype closely related to HELA
Viruses:
ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HPV +, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list