
SK-MEL-30
ACC 151
Cell line:
SK-MEL-30
DSMZ no.:
ACC 151
Species:
human (Homo sapiens)
Cell type:
melanoma
Origin:
derived from tumor tissue (subcutis metastasis) of a 67-year-old man with malignant melanoma in 1975
Reference(s):
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Medium:
90% RPMI 1640 + 10% h.i. FBS
Subculture:
seed out about 1 x 106 cells/80 cm2;
split confluent culture 1:4 to 1:10 about 2-3 times per week using trypsin/EDTA
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 30 hours
Harvest:
about 6-8 x 106 cells/80 cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology:
To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de.
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human hypertriploid karyotype with 3.5% polyploidy - 80(69-83)<3n>XXY-Y/XXYY, +1, +1, +2, +2, +3, +7, +10, -11, +12, +17, +18, +20, -21, +2mar, inv(6)(p11;q25), der(7)t(7;14)(p22;q22), der(9)t(9;?21)(p11;q11)x2, 14p+, der(15)t(15;?)(p12;?)x2, der(16)t(16;?)(q23;?), der(18)t(18;?)(p11;?), mar: ?der(19)t(1;19)(q12;q13) - additional subclonal rearrangements of ch : der(1)t(1;?)(p12;?) were present in a few cells
Molec. Genetics:
Genomic sequencing and/or ARMS assay show that the cell line carries the NRAS Q61K mutation (mu/wt), but no BRAF V600 mutation
Viruses:
ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
Delivery form | Prices | |
Frozen culture | 450,- € | |
Growing culture (please inquire for exact delivery time) | 900,- € | |
DNA isolated from cell line (25 µg) | 560,- € | |
DNA isolated from cell line (5 µg) | 140,- € |
see price list