
JOSK-I - Discontinued
ACC 155
Cell line:
JOSK-I - Discontinued
DSMZ no.:
ACC 155
Species:
human (Homo sapiens)
Cell type:
histiocytic lymphoma (cell line U-937)
Origin:
Caution: Cytogenetic and STR analyses established unequivocally that JOSK-I is genetically identical to human histiocytic lymphoma cell line U-937.
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Medium:
90% RPMI 1640 + 10% h.i. FBS
Subculture:
seed out at ca. 0.2-1.0 x 106 cells/ml and maintain at 0.3-1.0 x 106 cells/ml; split ratio of 1:2 to 1:5
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 35 hours
Harvest:
saturation density at about 1.5-2.0 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology:
CD3 -, CD4 +, CD13 +, CD14 -, CD15 +, CD19 -, CD33 +, CD68 +, HLA-DR +
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in the STR profile of cell line U-937.
Cytogenetics:
human hyperdiploid karyotype with 3.5% polyploidy - 56(52-56)<2n>Xder(X), +2, +4, +6, +7, +8, +12, -15, +17, +19, +20, +mar, der(X)t(X;3)(q11;p13)t(1;3)(q11;p25), del(1)(p11p22), t(1;5)(p21.1;q23), t(2;18)(p17;q23), del(4)(q22), add(6)(p23), del(7)(q21)/der(7)t(7;18)(q11.23;p11), der(10)t(10;11)(p14;q22.3)/der(10)t(10;11) (p14;q22.3)dup(10)(q25.1), der(11)t(10;11)(p14;q22.3), add(13)(p13), der(17)t(17;18)(p11;q12) - (mar resembles Ph) - carries uncommon 10;11 translocation associated with AML M5
Viruses:
ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
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