MHH-NB-11
ACC 157
Cell line:
MHH-NB-11
DSMZ no.:
ACC 157
Species:
human (Homo sapiens)
Cell type:
neuroblastoma
Origin:
established from a neuroblastoma metastasis at an adrenal site of a 4-year-old white boy in 1986; cells were described in the literature to express neuron-specific enolase and synaptophysin (but neither GFAP nor S-100 protein) and to carry a 20-fold amplification of MYCN (NMYC, N-myc)
Reference(s):
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Medium:
80-90% RPMI 1640 + 10-20% h.i. FBS + 2 mM L-glutamine + MEM non-essential amino acids
Subculture:
seed out at 1.5-3 x 106 cells/80 cm2; split confluent culture 1:2 to 1:4 every 4-6 days using trypsin/EDTA; a significant number of cells may detach spontaneously from the plastic surface and these floating cells may also be used for passaging. Note: medium must be changed frequently to prevent acidity which induces cell clumping; optimal dissociation after trypsination is affected by gentle aspiration with a #21 gauge needle and syringe
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 80-100 hours
Harvest:
cell harvest of about 10 x 106 cells/80 cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology:
To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de.
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human bimodal hyper-, diploid/tetraploid karyotype - 54(48-54)<2n>XY, +1, +1, +2, +6, +7, +8, +13, +20, der(1)t(1;?)(p32;?)x2, der(13)t(13;?2HSR)(q33;?2p23) - both the long HSR on ch 13 due to MYCN (NMYC, N-myc) amplification and the ch 1 short-arm rearrangements are typical of neuroblastoma - identity confirmed
Viruses:
ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list