KB-3-1 (subclone of HELA)
ACC 158
Cell line:
KB-3-1 (subclone of HELA)
DSMZ no.:
ACC 158
Species:
human (Homo sapiens)
Cell type:
cervix carcinoma (subclone of HELA)
Origin:
the cell line KB-3-1 was established as subclone of the parental KB cell line, which itself is infact a HELA subclone (shown by STR analysis). Cell line KB-3-1 was used to establish several drug resistance subclones, like the multidrug-resistant KB-V1 (DSMZ ACC 149) resistant to vinblastine. KB-3-1 is kept in the open collection of DSMZ as parental clone of KB-V1 and the original HELA as DSMZ ACC 57
Biosafety level:
1
Risk assessment:
The cell line is infected with papilloma virus type 18 (HPV-18). The cells contain several copies as proviruses integrated into the eukaryotic genome. The integrated virus genomes are incomplete and exhibit 2-3 kb deletions of the E2-L2 region. An activation and transmission of the HPV-18 during handling of the cell line is improbable. The cell line is categorized biosafety level 1.
Permissions and restrictions:
Depositor-specific restrictions:
The cell line will only be distributed to non-profit institutions,
commercial entities may apply for a license at the NIH/NCI Technology Transfer Center, Rockville, MD, USA, phone (301)435-3111. Transfer to third parties needs to be negotiated with the depositor.
DSMZ Cell Culture Data:
Morphology:
Medium:
90% DMEM + 10% h.i. FBS + 1 mM sodium pyruvate
Subculture:
split confluent culture up to 1:10 using trypsin/EDTA; seed out at ca. 1 x 106 cells/25 cm2
Incubation:
at 37 °C with 5% CO2
Doubling time:
about 40 hours
Harvest:
about 6-10 x 106 cells/80 cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology:
To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de.
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of HELA in the reference STR database.
Cytogenetics:
human hypertriploid karyotype with 1.8% polyploidy - 76(73-78)<3n>XX, -X, -1, -3, -4, +6, +7, +8, -9, -18, -20, +11mar, der(1)t(1;?)((p11;?), der(3)t(3;?)(p11;?), der(3) t(3;?)(p14;?), der(3p;5q), del(6)(q21), del(7)(q31), der(8)t(8;?11) (q24;q11), der(9) t(9;?)(q34;?), der(12)t(12;?)(p12;?), der(19)t(19;?)(q13;?) - supernumerary single dm present in 11% cells - 3 or more HELA markers present
Viruses:
ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HPV +, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list