BL-41
ACC 160
Cell line:
BL-41
DSMZ no.:
ACC 160
Species:
human (Homo sapiens)
Cell type:
Burkitt lymphoma
Origin:
established from the tumor tissue of an 8-year-old boy with Burkitt lymphoma; cells were described in the literature to be EBV-negative
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Medium:
80-90% RPMI 1640 + 10-20% h.i. FBS (possibly add 1 mM sodium pyruvate + 50 µM mercaptoethanol)
Subculture:
seed out at ca. 0.5 x 106 cells/ml (initially in a 24-well-plate); split saturated culture 1:3 to 1:5 every 2 days; optimal density at about 1.0 x 106 cells/ml
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 30 hours
Harvest:
saturation density at about 2.0 x 106cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology:
CD3 -, CD13 -, CD19 +, CD20 +, CD34 -, CD37 +, CD38 +, cyCD79a (+), CD138 +, HLA-DR +, sm/cyIgG -, sm/cyIgM +, sm/cykappa +, sm/cylambda -; image
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human hyperdiploid karyotype with 7% polyploidy - 48(42-49)<2n>XY, +7, -13, +2mar, add(8)(q24), t(8;14)(q24;q32), der(15)t(13;15)(q13;p11), add(17)(p12), subclonal rearrangements at 1q23, 7p22, 11q13
Viruses:
ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list