HELA-S3 (subclone of HELA)
ACC 161
Cell line:
HELA-S3 (subclone of HELA)
DSMZ no.:
ACC 161
Species:
human (Homo sapiens)
Cell type:
cervix carcinoma (cell line HELA)
Origin:
the cell line HELA-S3 is an early subclone of HELA. It was established in 1955 by single cell cloning of the parental HELA cell line and is described to exhibit a more uniform morphology than the parental cell line; the cells were further described to can be grown in suspension (but no more in our hands). The original HELA is available as DSMZ ACC 57
Biosafety level:
1
Risk assessment:
The cell line is infected with papilloma virus type 18 (HPV-18). The cells contain several copies as proviruses integrated into the eukaryotic genome. The integrated virus genomes are incomplete and exhibit 2-3 kb deletions of the E2-L2 region. An activation and transmission of the HPV-18 during handling of the cell line is improbable. The cell line is categorized biosafety level 1.
Permissions and restrictions:
DSMZ Cell Culture Data:
Medium:
90% Ham's F12 + 10% h.i. FBS
Subculture:
split confluent culture 1:5 to 1:8 every 3-4 days using trypsin/EDTA; seed out at ca. 2-3 x 106 cells/80 cm2
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 30-40 hours
Harvest:
harvest of about 7-10 x 106 cells/80 cm2 flask
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology:
To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de.
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of HELA cell line in the reference STR database.
Cytogenetics:
human flat-moded hypotriploid karyotype with 32% polyploidy - 68(53-68)<3n>XX, -X, +1, -2, -4, +5, +8, +12, -13, -14, -20, -21, -22, -22, +4mar - del(1)(p35), der(1)t(1;3) (p11;q11), der(3)t(3;5)(q11;q11), i(5p)x2, del(6)(q23), add(6)(q27), del(7)(q32), i(8q), add(8)(q24), del(9)(p22p23), t(11;19)(q13;q13), add(12)(q23)x2, add(13)(p12), i(16q), add(19)(p13), add(21)(p11), i(22q) - high level of spontaneous aberrations - matches published karyotype
Viruses:
ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HPV +, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list