PA-TU-8988T
ACC 162
Cell line:
PA-TU-8988T
DSMZ no.:
ACC 162
Species:
human (Homo sapiens)
Cell type:
pancreas adenocarcinoma
Origin:
established in 1985 from the liver metastasis of a primary pancreatic adenocarcinoma from a 64-year-old woman; described to carry mutations in KRAS, SMAD4 and TP53;
sister cell line of PA-TU-8988S (DSMZ ACC 204)
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Medium:
90% DMEM + 5% h.i. FBS + 5% horse serum + 2 mM L-glutamine (some researcher cultivate also with horse serum)
Subculture:
seed out at ca. 0.5 x 106 cells/80 cm2; split confluent culture 1:5 to 1:10 every 3-5 days using trypsin/EDTA
Incubation:
at 37 °C with 5-10% CO2
Doubling time:
about 25-30 hours
Harvest:
cell harvest of about 20 x 106 cells/80 cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology:
To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de.
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human near-triploid karyotype with 2% polyploidy - 68(63-70)<3n>XXX, -4, +6, +6, +8, +11, -15, -16, -17, -19, +20, -21, -22, +mar, der(1)t(1;?)(p11;?), i(6p), del(6)(q23)x2, der(8)t(8;?)(q24.3;?), der(8)t(8;?)(p11;?)x2, der(11)t(11;?)(p13;?)x2, del(12)(p12.3), dup(14(q11q12), del(17)(p12), der(17)t(17;?)(p13;?) - sideline with additional der(3)t(3;?)(q11;?) - 1p11 and 8q24 breakpoints previously reported in pancreatic cancer
Viruses:
ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list