NCI-H929

human (Homo sapiens)

multiple myeloma

established from the pleural effusion of a 62-year-old white woman with myeloma (IgAkappa) at relapse; cells were described as follows: cells synthesize high amounts of immunoglobulin, MYC proto-oncogene is rearranged and MYC RNA is expressed, cells are EBV-negative; patented cell line

14549, 14486

1

A

single, round to oval cells or clustered in small clumps in suspension; image; image; image

80-90% RPMI 1640 + 10-20% h.i. FBS + 2 mM L-glutamine + 1 mM sodium pyruvate + 50 µM mercaptoethanol

cells are very difficult to culture; seed out at 1 x 10⁶ cells/ml; cultivation in 12- or 24-well plates is highly recommended; maintain at a minimum of 1 x 10⁶ cells/ml; after thawing cells grow very slowly and viability may be initially low (possibly no growth during the first week); arising debris may mimick a bacterial contamination

at 37 °C with 5% CO₂

ca. 70-80 hours

maximum density at 1.5 x 10⁶ cells/ml

frozen with 70% medium, 20% FBS, 10% DMSO

negative in DAPI, microbiological culture, RNA hybridization, PCR assays

CD3 -, CD10 -, CD13 -, CD19 -, CD20 -, CD34 -, CD38 +, CD80 -, CD138 +, HLA-DR -, cyIgG -, cyIgM -, cykappa +, cylambda -; image

STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.

human hypodiploid karyotype with 16% polyploidy - 45(43-46)<2n>X, -X, +8, -10, -13, +mar, dup(1)(q11q25), del(1)(p11p25), del(6)(q25), der(8)t(8;?)(q23;?)x2, del(9)(p13), del(12)(p12)

ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -

Delivery form		Prices
Frozen culture		450,- €
Growing culture   (please inquire for exact delivery time)		900,- €
DNA isolated from cell line (25 µg)		560,- €
DNA isolated from cell line (5 µg)		140,- €

see price list

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