
IMR-32
ACC 165
Cell line:
IMR-32
DSMZ no.:
ACC 165
Species:
human (Homo sapiens)
Cell type:
neuroblastoma
Origin:
established from an abdominal mass from an untreated neuroblastoma (supposedly N-type) of a 1-year-old boy in 1967; described to carry amplyfied MYCN; widely used and extensively characterized cell line; listed in the CCLE (ref 18232)
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Medium:
80% RPMI 1640 + 20% h.i. FBS + 1x non-essential amino acids
Subculture:
seed out at ca. 0.5-1.0 x 106 cells/80 cm2; split confluent culture 1:2 to 1:4 every 3-5 days (trypsin need not be used, cells can be detached by tapping the flask or pipetting)
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 40-50 hours
Harvest:
about 5-10 x 106 cells/80 cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology:
To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de.
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human hyperdiploid karyotype - 49(44-51)<2n>XY, +1, +6, +12, der(1)t(1qter->1p34::hsr::17q22->qter)x2, add(16)(q24) - sideline with del(3)(p14), add(7)(q35), del(9)(q34), add(19)(q13) - NB associated changes include: N-myc amplified in hsr regions and unbalanced t(1;17) - matches published karyotype
Viruses:
ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
Delivery form | Prices | |
Frozen culture | 450,- € | |
Growing culture (please inquire for exact delivery time) | 900,- € | |
DNA isolated from cell line (25 µg) | 560,- € | |
DNA isolated from cell line (5 µg) | 140,- € |
see price list