Species:
human (Homo sapiens)
Cell type:
chronic myeloid leukemia in blast crisis
Origin:
established from the peripheral blood of a 29-year-old woman with chronic myeloid leukemia (CML) (treated 5 years with busulfan from 1979-1984) one month after onset of myeloid-megakaryocytic blast crisis; cells were described in the literature to express megakaryocytic and erythroid markers and to respond to induction of differentiation with various reagents; parental cell line of subclone LAMA-87 (DSM ACC 270); cells carry t(9;22) leading to BCR-ABL1 e14-a2 (b3-a2) fusion gene. Exome and RNA sequence data are available (see Ref 18187 and
Exome sequence and
RNA-Seq)
Permissions and restrictions:
Morphology:
single, round to polymorph cells growing in suspension, some cells are loosely adherent with a spindle-like morphology;
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Medium:
90% RPMI 1640 + 10% h.i. FBS
Subculture:
maintain at 0.5-1.0 x 106 cells/ml; optimal split ratio of 1:2 to 1:3 every 2-4 days; dislodge any adhering cells by shaking culture flask; seed out at ca. 0.5 x 106 cells/ml
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 50 hours
Harvest:
saturation density at about 2.0 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology:
CD3 -, CD11b +, CD13 +, CD15 -, CD19 -, CD33 +;
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Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human hypertriploid karyotype with 3.6% polyploidy - 73/74(69-77)<3n>XX, -X, +1, -2, +5, +6, +8, +13, -14, +17, +17, -18, +22, +mar, del(7)(p15), der(9)t(9;22)(q34;q11)x2, i(11q), add(13)(q33), del(17)(p12), der(22)t(9;22)(q34;q11)x4 - carries Ph (4 copies) - resembles published karyotype
Molec. Genetics:
expression of fusion gene BCR-ABL1 e14-a2 (b3-a2) confirmed by RT-PCR
Viruses:
ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
| Delivery form | | Prices |
|
| Frozen culture | | 450,- € |
Growing culture
(please inquire for exact delivery time) | | 900,- € |
| DNA isolated from cell line (25 µg) | | 560,- € |
| DNA isolated from cell line (5 µg) | | 140,- € |
see price list
